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STUDY OF ENZYMATIC ACTIVITY OF BACILLUS SUBTILIS MICROORGANISMS STRAINS CULTIVATED WITH USE OF BEER PELLET

13 December 2016
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Authors: AmangeldiyevaD.
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SpeciesBacillusattractthe attention of researchersfor a long time. The accumulated knowledgeinthe field of microbiology, physiology, biochemistry, and geneticsof bacteria testifies about benefits of Bacillusas a producers of biologically activesubstances:enzymes, antibiotics, insecticides [1-4].Highadaptability to variousconditions of existence(presence orabsence of oxygen, the growth and development ina considerable range oftemperatures, the use of various organic orinorganiccompounds, etc. as sourcesof nourishment) contribute to the spread ofgermsin the soil,water, air, foodstuffsand otherenvironmental objects, as well as in humans andanimals.

A variety of metabolic processes, genetic and biochemical variability, resistance to lytic and digestive enzymes, have served as justification for the use of bacilli in the various fields of medicine [5-8].

Analysis of the results of research carried out in our country and abroad, testifies about scales of use of the Bacillus bacteria to produce products from biomass of bacteria or their metabolites. Known methods for culturing bacteria of the species Bacillus are the basis for the technology of obtainment of a number of bacterial and enzyme preparations [9-12].

The aimof this work isselectionof microorganismBacillus subtilisstrainfrom soilof South Kazakhstan regionandstudythe complexmetabolite, the metaboliteactivity of isolated strain, aswell asthe hydrolyticactivity and biological properties ofisolated strains ofB.subtilisand to evaluate the possibilityof their usefor the developmentof the originalbiological product.

Creation of optimal conditionsfor the growthof bacteriaBacillus subtilis have essentialmeaning for the creationof biological products ontheir basis.The medium was prepared by weighing the following medium composition in grams per litre; Bacteriological peptone-6g, MgSO4.7H2O-0.5g, KCL-0.5g, substrate-1.0g. The above medium composition were dissolved in 1000ml of distilled water after which 100ml of the medium was measured into a conical flask (250ml capacity each) heated on hot plate to homogenize and then sterilized in an autoclave at 121℃ for 15 minutes after which they were removed and allowed to cool before the organism was inoculated.The screening of amilolytic activity of  B. Subtilis were used Louria-Bertoni medium with 1% of starch solution.Hydrolytical activity is determined in supernatant from cultures, which are obtained by centrifugation of all volume of culture. Asapolysaccharide substrates are used potato starch, also extract of beer pellet.For estimation of proteolytic activity were used hydrolysis of 1% casein solution by proteases.

Bibliography and references
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